Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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DNA MARKERS FOR RESISTANCE TO DOWNY MILDEW (PLASMOPARA HALSTEDII) IN WILD SUNFLOWER

Usatov A.V. 1 Azarin K.V. 1 Tikhobaeva V. E. 1 Volichenko M.I. 1 Gavrilova V.A. 2 Markin N.V. 1
1 Southern federal university
2 N.I. Vavilov Research Institute of Plant Industry
A search for new donor genes for resistance Downy mildew (Plasmopara halstedii) among annual and perennial wild species of Helianthus L was conducted. It is shown that the identification of the resistance locus Pl 6 in annual species is informative marker На Р2 (1200 bp), while in perennial species - На Р3 (1800 bp). At the same time it should be noted that for perennial species in most cases amplification of DNA with a primer На Р2 coincide with the results of PCR analysis with primer На Р2. Discovered on the results of molecular genetic analysis a wild forms, containing the locus Pl 6, are of direct interest for the breeding of cultivated sunflower.