Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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THE DEHYDROGENASE ACTIVITY OF SULFATE-REDUCING BACTERIA AS A PARAMETER OF REAGENT EFFECTIVENESS VALUE IN OIL INDUSTRY

Drogaleva T.V. 1, 2 Abdrashitova J.N. 2 Kolokolova N.N. 1 Bome N.A. 1
1 Tyumen State University
2 Gyprotyumenneftegaz
The comparative results of biocide effectiveness determination of 7 reagents with different active components in relation to sulfate-reducing bacteria (SRB) selected from waters of the Ust-Teguss oil field are presented. The biocide effectiveness of reagents was determined by two ways: by the method used at the present moment for the value of oil industry bactericide effectiveness (RD 39-3-973-83) and by change of the relative dehydrogenase activity of sulfate- reducing bacteria. The synthetic model of formation water (the source of selected SRB) with sodium lactate as an electron donor is proposed as a medium for the bacteria contact with the reagents. The similar results of biocide effectiveness determination of reagents by two different methods are obtained. The possibility of development a new method of biocide effectiveness determination of reagents based on measurement dehydrogenase activity of sulfate- reducing bacteria is shown. The evolution of dehydrogenase activity of sulfate-reducing bacteria is studied, it is shown their highest activity on the 2nd-3rd incubation days.