Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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DETECTION OF NULL WX-B1B-ALLELE OF THE WAXY-GENE IN SPRING WHEAT GENOTYPES OF RUSSIAN BREEDING

Abdulina I.R. 1, 2 Vafin R.R. 1 Tyulkin S.V. 2 Zaynullin L.I. 1 Alimova F.K. 1 Askhadullin D.F. 3, 3 Vasilova N.Z. 3
1 Kazan (Volga region) federal university
2 Tatar trans-regional veterinarian laboratory
3 Tatar research institute of agriculture of RAAS
The approbation of the well-known and developed by us PCR methods for the identification of allele variants of Wx-B1-locus of wheat Waxy-gene was performed on 70 spring wheat samples from breeding of the Tatar research institute of agriculture/. A distinctive feature of the developed PCR method from the prototype is the use instead of primer Wx-B1F an oligonucleotide 4F-c, which generates, compared to the prototype, reduced by 61 bp PCR products in length for 402 bp (Wx-B1a-allele) and 436 bp (Wx-B1e-allele), ensuring a better separation of amplified fragments in agarose gel, and, accordingly, increase the accuracy of interpretation of the results of genotyping. Designed and optimized by us PCR methods for the identification of allele variants of Wx-B1-locus of wheat Waxy-gene and approbated on spring wheat samples from breeding of the Tatar research institute of agriculture allowed to perform a correct identification of the studied Triticum aestivum genotypes with identection two economically valuable lines that carry in their genomes null Wx-B1b-allele.