Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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INFORMATIVE DNA MARKERS OF GENE RF1 - POLLEN FERTILITY RESTORER CMS PET1 IN SUNFLOWER

Markin N.V. 1 Usatenko T.V. 2 Usatov A.V. 1 Tikhobaeva V.E. 1 Gorbachenko O.F. 2 Kulishova G.A. 1 Azarin K.V. 1
1 Southern federal university
2 Don Experimental Station L.A. Zhdanov VNIIMK
On the breeding material - 17 CMS lines and 29 Rf-lines Don Experiment Station LA Zhdanov VNIIMK was study 9 DNA markers - ORS 511, ORS 224, ORS 317, ORS 630, ORS 799, ORS, 1030, HRG01, HRG02 and STS-115, which with the gene Rfl - pollen fertility restorer CMS PET1 form a common linkage group. Three markers - HRG01, HRG02 and STS-115 were highly informative for identifying gene Rfl. Based on these results, it was to develop a multiplex PCR test-system for the detection of nuclear marker of gene Rfl and mitochondrial locus orfH522 (CMS PET1). This test-system can be used in sunflower breeding.