Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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APPLICATION OF BARLEY DOUBLED HAPLOIDS IN GENETICS AND BREEDING

Mishutkina Y.V. 1 Neskorodov Y.B. 1 Novokreshchenova M.G. 2 Malakho S.G. 2 Touraev A.М. 2
1 Centre “Bioengineering” RAS
2 Lomonosov Moscow State University
Doubled haploid barley is widely used during last 30 years in barely breeding programmes and in fundamental-applied research. First haploid barley plants were obtained using chromosome elimination during distance hybridization with Hordeum bulbosum (Bulbosum method). This technology is still in use in breeding programs of several companies. Later the method of isolating and culturing in vitro anthers and microspores were established as an efficient technology of doubled haploid production in barley. Nowadays barley can be evaluated as a model cereal for haploid and doubled haploid studies. Doubled haploids were used for creating molecular markers and chromosome mapping. Isolated barley microspore cultures in combination with molecular biology and genomics methods led to studies of the mechanism of microspore embryogenesis and doubled haploid formation. Application of haploid microspores as target for genetic transformation led to the generation of homozygous transgenics already in the first generation.