Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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PRODUCTION OF DOUBLE HAPLOIDS IN DOMESTIC VARIETIES OF BRASSICA NAPUS VIA ISOLATED MICROSPORE CULTURES

Neskorodov Y.B. 1 Mishutkina Y.V. 1 Kabardaeva K.V. 1 Touraev A.M. 2
1 Centre “Bioengineering” RAS
2 Lomonosov Moscow State University
The aim of this research was the comparison and evaluation of two established earlier methods of obtaining doubled haploid rapeseed, classical microspore embryogenesis and embryogenesis via the formation of suspensor like structures, in various Russian varieties. We did not observe significant differences in the frequency of embryo formation and regeneration of plants between two protocols. Thus, both methods can be used to obtain doubled haploid plants in tested Russian varieties. The frequency of embryo formation and regeneration of plants were a little lower compared to the control variety, Brassica napus cv. Topas, which is known to be the most effective variety for doubled haploid production via microspore cultures in rapeseed. The frequency of regenerated plants from formed embryos was from 10% till 58% in classical methods and, from 18 till 69% - in the methods, based on the use of suspensor like structures, whereas the frequency of regeneration in Topas reached 61%. The total number of regenerated plants was enough to use both methods to obtain necessary number of doubled haploids in all varieties, tested for the use in the breeding programs.