Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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THE REVIEW OF KNOWN WAYS OF OBTAINING RECOMBINANT HUMAN LACTOFERRIN WITH EUKARYOTICAND BACTERIAL CELLULAR SYSTEMS

Novoselova M.V. 1 Linnik A.I. 1 Dyshlyuk L.S. 1 Matskova L.V. 2
1 Kemerovo Technological Institute of Food Industry
2 Karolinska Institute
The analysis of known systems of production recombinant lactoferrin is carried out: microscopic mushrooms, plants (tobacco, potatoes, rice), transgene animals (cows, goats), microorganisms. Merits and demerits of known genetically engineered ways of obtaininghuman lactoferrin are revealed. The main demerits of existing methods are labor input of process, complexity of cleaning, decrease in biological activity of protein, a low exit of a target product. E. TOli strain as a perspective producer of recombinant human lactoferrin is chosen. The scheme of cloning of ltf geneis offered. Own researches on obtaining recombinant human lactoferrinare conducted. The expression of lactoferrinis proved at all used types of secretion. Optimum parameters of cultivation of recombinant strain E. wli, allowing to obtain the maximum exit of protein are chosen.