Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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CHARACTERISTICS OF PROTEINS ASSOCIATED WITH FROST HARDINESS DEVELOPMENT IN TWO POPULATIONS OF BETULA PLATYPHYLLA OF YAKUTIA

Ponomarev A.G. 1 Tatarinova T.D. 1 Perk A.A. 1 Vasilieva I.V. 1 Bubyakina V.V. 1
1 Institute of Biological Problems of Cryolithozone
Intraspecific variability of total proteins and dehydrins in buds of Betula platyphylla Sukacz. experiencing extremely cold climatic conditions of Eastern Siberia was studied by SDS-electrophoresis in PAAG and western immunoblot analysis. Two variable regions (14-16 and 22-25 kD) in total protein spectra and two groups of low (15-21 kD) and medium (56-73 kD) molecular mass dehydrins were found in Central and Southern Yakutia populations of birches. Polymorphism of dehydrins with molecular masses 15-21 kD was greater in Central Yakutia population than in Southern Yakutia one. The dehydrin of 17 kD occurred in all birches of both populations. Dehydrins of 15-21 kD supposedly associated with the frost hardiness had high stable level in winter and were not identified in growing season in Central Yakutia population of birches. Dehydrins of 56-73 kD were not quantitatively different in both populations and were detected all year round, decreasing in summer.