Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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EXPRESS SYSTEM FOR SCREENING OF COMPOUNDS PREVENTING BINDING OF HUMAN IMMUNODEFICIENCY VIRUS (HIV) TO SENSITIVE CELLS

Prokofjeva M.M. 1 Orlova N. N. 1, 2 Stepanov O.A. 2 Nikitenko N.A. 1 Gornostaeva A.S. 1, 2 Lebedev T.D. 1 Klimova A.N. 1, 2 Burnysheva K.M. 1 Mitkevich V. A. 1 Spirin P.V. 1 Prassolov V.S. 1, 2
1 Engelhardt Institute of Molecular Biology RAS
2 Moscow Institute of Physics and Technology (State University)
Development of the efficient cell systems for drug screening against HIV is crucial for the creation of new antiviral therapeutic agents. The inhibitors of virus binding to host cell, thus preventing spread of infection, represent a great interest. We describe system based on recombinant lentiviral vectors for screening potential antiviral compounds that prevent the initial non-specific binding of HIV to the heparan sulfates on target cells surface. We have studied the inhibitory activity of a number of sulfated polysaccharides that prevent non-specific binding of lentiviral particles to the target cells. It has been shown that sulfated polysaccharides inhibit lentiviral transduction of T-lymphoblastic Jurkat cell line by lentiviral particles carrying HIV envelope protein on their surface.