Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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IMMUNOCHROMATOGRAPHIC SERODIAGNOSYS OF CATTLE BRUCELLOSIS WITH THE USE OF COLLOIDAL GOLD - BRUCELLA ABORTUS LIPOPOLYSACCHARIDE CONJUGATE

Sotnikov D.V. 1
1 A.N. Bach Institute of biochemistry
In this paper the method of immunochromatographic analysis applied for serodiagnosis (detection of specific antibodies) of cattle brucellosis is studied. A distinctive feature of the proposed method is the use of colloidal gold - Brucella abortus lipopolysaccharide (LPS) conjugate and the nitrocellulose membrane with immobilized Brucella abortus LPS (instead of antispecies antibodies used in traditional immunochromatographic test-systems for serodiagnosis). Due to several antibody valences a simultaneous binding between gold-labeled LPS and LPS immobilized on a membrane strip with the formation of colored area in analytical zone occurs. This approach allows the detection of the minimum concentration of specific antibodies against more than 90% excess of nonspecific antibodies eliminating the influence of the latter on the assay results. The efficiency of the proposed approach was confirmed by the testing of 39 cows. The results of the proposed assay correlate well with the data of traditional immunoenzyme assay.