Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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SPECTRAL ANALYSIS OF HEMOGLOBIN BY MICROORGANISMS WITH DIFFERENT LEVELS OF ANTI - HEMOGLOBIN ACTIVITY

Schuplova E.A. 1 Fadeev S.B. 1
1 The institute for cellular and intracellular symbiosis
The aim - spectral analysis of hemoglobins structure by microbial action with different levels antihemoglobin activity. Materials and methods. The optical spectra of the samples (scanning spectrophotometer “Genesys 5”, USA), including 2 ml of the supernatant of daily culture of microorganisms strains and 0.5 ml suspension of washed donor human erythrocytes (concentration 106 cells / ml) 2h, 6h and 24h of preparation said sample were measured. 30 strains of Staphylococcus with antihemoglobin activity, was used in this study. Results. The decrease of the optical density of samples containing red blood cells hemolysate, in the range of 220-450 nm was influenced by the supernatants of strains of Staphylococcus. The most expressed decrease in the optical density under the supernatansts of strains with high antigemoglobin activity (p <0.05), especially in the range of 398-422 nm (porphyrin pocket) were detected. This indicates that staphylococcus high AntiHbA violate the protein structure of hemoglobin more than microorganisms with low activity. In the ranges of500-700 nm, characteristic of the heme part of hemoglobin, increased optical density of the test samples compared to control was founded. The largest increase in this index at a wavelength of 619 nm under the influence of strains with high AntiHbA, (p <0.05) were detected. This indicates the increase in the proportion of methemoglobin in 3.3 times (to 13%). Conclusion. Spectral analysis of the structure of hemoglobin by microbial action with different levels of antighemoglobin activity revealed that bacterial products can damage the conformational structure of the protein component of hemoglobin, as well as affect the heme, increasing the proportion of methemoglobin.