Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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STRUCTURE OF RHIZOSPHERAS BIOFILM OF CUCURBITA PEPO L.

Artamonova M.N. 1 Pchelintseva Е.S. 2 Kostishko B.B. 2 Potaturkina- Nesterova N.I. 1
1 Ulyanovsk State University
2 Laboratory scanning probe and electron microscopy of Research Technology institute of Ulyanovsk State University
It has been studied the morphology of rhizobacteria biofilms of pumpkin with atomic force microscopy. It has been used semicontact method and method mismatch. Atomic force microscopy allowed to study the structure of the rhizobacteria biofilm. It has been founded that the main components of the rhizosphere biofilm were bacteria and matrix. The thickness of the matrix was 350 nm, which was about 99 % of the total thickness of the biofilm. Rhizobacteria were like a rod-shaped cells, the mean size was 2,39 ± 0,07 nm (length ), 1,37 ± 0,09 nm (width ), 1,35 ± 0,08 nm (height) . Adhesive capacity of the matrix was significantly less than the adhesive activity of rhizobacteria – and this index was 8,3 ± 0,6 nH and 49,2 ± 1,1 nH, respectively (p <0.05). 3 D- images revealed that the biofilm surface was not smooth and had many irregularities. Profile of the phase shift in a selected area image allowed to research the spatial organization of the rhizobacteria biofilm.