Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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RESEARCH OF ABILITY OF LISTERIA MONOCYTOGENES TO FORM BIOFILMS IN CONSORTIUM WITH SAPROPHYTIC BACTERIA

Bogatyrenko Е.А. 1, 2 Buzoleva L.S. 1, 2 Berdasova A.S. 1
1 Far Eastern Federal University
2 Research institute of epidemiology and microbiology n.a. G.P. Somov
For research of ability of pathogenic bacteria to form biofilms in interaction with saprophytic microorganisms the strains of Listeria monocytogenes and strains of saprophytes isolated from various food were used. The work was carried out with the modified method based on a photometric estimation of quantity of 1% solution of the crystal violet joined with a biofilm. Experiment was carried out at 5 ºС, 22ºС and 37ºС. It is shown that at 37ºС L. monocytogenes effectively formed biofilms in a monoculture, however at 5ºС and 22ºС this ability was lost. Presence of saprophytes in medium with L. monocytogenes had ambiguous effect on biofilm formation process. So, at 37ºС saprophytes rendered the action both stimulating and inhibiting on formation of biofilms. However, at temperatures of 5ºС and 22ºС all studied saprophytic microorganisms positively influenced formation of biofilms with pathogenic bacteria. It is established that the most effective formation of biofilms of L. monocytogenes and saprophytes was observed at 22 ºС.