Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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BIOCHEMICAL ADAPTATIONS OF SAPROZOONOSIS AGENTS TO ENVIRONMENTAL FACTORS

Buzoleva L.S. 1, 2 Krivosheeva A.M. 2 Bogatyrenko Е.А. 1, 2 Sinelnikova M.A. 3
1 Far Eastern Federal University
2 Research institute of epidemiology and microbiology n.a. G.P. Somov
3 Primorsky state agricultural academy
For studying of adaptations mechanisms providing possibility of pathogenic bacteria reproduction in objects of environment the strains Listeria monocytogenes and Yersinia pseudotuberculosis were cultivated on different mineral and organic mediums at temperatures 6-8 °C, 18-20 °C and 37 °C. It is shown that at a low temperature (6-8 °C) at first the delay of bacteria reproduction was observed, but then the curve of growth reached the same indicators as at 37 °C. Thus the evidence of reproduction, but not surviving of the studied cultures was obtained at a low temperature. Hydrolysis of acetyltiocholin by Y. pseudotuberculosis at low temperatures increased in comparison with 37 °C. Existence of conformational enzymes changes of studied cultures with changing of temperature conditions is shown. Low temperature cultivation of L. monocytogenes и Y. pseudotuberculosis increased synthesis of NAD and NADPH in 1,5-2 times and RNA for 7-8% in comparison with cultivation of bacteria at 37 °C. Y. pseudotuberculosis cultivated on mineral and organic mediums in the conditions of low temperature had more intensive accumulation of nonsaturated fatty acids in membranes than at 37 °C