Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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THE ANALYSIS OF SENSITIVITY AND DIAGNOSTIC EFFICACY OF THE TEST SYSTEM OF INDICATION AND IDENTIFICATION OF BACTERIA B.BRONCHISEPTICA

Vasilyeva Y.B. 1 Mastilenco A.V. 1 Vasilyev D.A. 1 Badaev R.R. 2 Merhcina S.V. 1 Shvidenko I.G. 3 Skorik A.S. 1
1 FSBEI HPE “Ulyanovsk state agricultural Academy named after P.A. Stolypin”
2 Research financial law University
3 FSBEI HPE “Saratov agrarian University named after N.I. Vavilov”
The paper presents results of a study of analytical sensitivity and diagnostic efficacy of the test system of indication and identification of bacteria B.bronchiseptica (TSII BBR). TSII BBR includes bacteriological, immunological, molecular genetics and phage components, provides early and accurate diagnosis of Bordetella. The analytical sensitivity of bacteriological and immunological components of the test system is not less than 104 microbial cells in ml, molecular genetics and phage - not less than 103 microbial cells in ml. Bacteriological, immunological, phage components should be used in the early stages of the disease (not later than the third week), before treatment with antibacterial drugs. At a later date and on the background of antibiotic therapy inoculation significantly reduced. PCR is more effective in the later stages of the disease and on treatment with antibiotics, but the maximum efficiency of the method on 1 - 3 week from the onset of the disease. Antibiotics may lead to false-negative result of the analysis. The term laboratory tests using the test system of indication and identification of bacteria B.bronchiseptica is from 1 to 4 days.