Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

Personal portfolio

INFLUENCE OF THE COMPOSITION OF COLLOIDAL GOLD - PROTEIN CONJUGATES ON THE EFFICIENCY OF THEIR APPLICATION IN IMMUNOCHROMATOGRAPHIC ASSAYS

Byzova N.A. 1 Sotnikov D.V. 1
1 A. N. Bach Institute of Biochemistry
The article discusses the problem of optimal composition for conjugates between proteins and colloidal gold used in immunochrimatographic assay. Traditional recommendations for choosing the optimal composition of the colloidal conjugates suggest using the flocculation method, i.e. the study of colloidal gold - protein complexes stabilization in a solution in high ionic strength. The dependence of the optical density of the colloidal conjugate on the concentration of the immobilized protein reaches a plateau, indicating stabilization of the surface. Flocculation curves and binding immunochromatographic data were compared for the cognates of different composition of colloidal gold with immunoglobulin G, staphylococcal protein A and streptococcal protein G - basic reagents used in immunochromatography. The differences of the experimental dependences for different proteins were demonstrated. Thus, for achieving the maximum binding of the assay for protein G requires twice the concentration of immobilized protein (8 ug/ml) than follows from the flocculation curve (4 ug/ml).