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Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454
ISSN 2500-3399
ПИ №ФС77-57454
FEATURE COMPARISON OF METHODS FOR DIAGNOSTICS OF BORDETELLOSIS
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The article presents a comparative analysis of instructional techniques identify Bordetella bronchiseptica. The studies found that the bacteriological method is effective and has a relatively high specificity, but its implementation is a costly, laborintensive and time (96-120 hours). Immunological methods distinguished by the simplicity and speed of execution, but they are not specific enough, and with their help it is impossible to distinguish current infection from the past or from the carrier. Molecular genetic techniques, being a reliable, rapid, highly sensitive and specific, allow for direct detection of Bordetella, select the agent among closely related bacteria that do not require a response to the viability of the parasites. The drawback is the high cost of analyzes, multiple stages and the possibility of false-positive and false-negative results due to contamination of the material or human error. Method method for diagnosis of phages has a high specificity for the final diagnosis is spent up to 66 hours, the method is economical compared to the bacteriological examination. Reaction Rise phage titer can detect Bordetella at a concentration of 103 MK in 1 ml of the test material for 26 hours. To select an effective method of diagnostic of bordetellosis or their combined use, consider the following criteria: extent of the planned research, the period of infection cycle, the contingent animals, their immune status.