Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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EFFECT OF VARIOUS CONCENTRATIONS OF NITROGEN OXIDE DONOR SNP ON ZEIDEL ASCITES HEPATOMA CELLS SURVIVAL IN VITRO

Kupriyanova E.S. 1, 2 Naumov A.A. 1, 2 Serebryakova L.T. 2 Potselueva M.M. 1, 2
1 Pushchino State Institute of Natural Sciences. (142290
2 Institute of Theoretical and Experimental Biophysics RAS (142290
One of the key areas of modern science is the study of mechanisms of occurrence, development and treatment of malignant neoplasms. Improving methods of chemotherapy requires intensive study of the mechanisms of action of new cytotoxic chemicals. At present studies, the key role in the regression of tumors is assigned to reactive oxygen species. The purpose of this study was to investigate the effect of various concentrations (0,02 mМ to 2 mМ) of the nitrogen oxide donor – sodium nitroprusside (SNP) on the growth of Zeidel ascites hepatoma cells. It was found that SNP produces a cytotoxic dose-dependent effect (action) to the cells of this type under conditions of in vitro cultivation. However, SNP concentrations below 0.2 mM are favourable for proliferation of Zeidel ascites hepatoma cells, which was recognized by analysis of cell cycle phases by the method of cytofluorometry.