Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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AMINO ACIDS MODIFICATIONS DETECTION WITH MALDI-SPECTROSCOPY

Lapshin G.D. 1 Vinokurov L.M. 2 Savitsky A.P. 1
1 A.N. Bach Institute of biochemistry RAS
2 Branch of Semyakin & Ovchinnikov Institute
Chemical amino acids modification is popular method of modification of protein properties. Amino acid modification is stochastic process. Identification of the exact spot of amino acid modification in protein sequence is complex task. Information of amino acid modification position could be used with further direct mutagenesis of an amino acid in this position. Method of amino acid identification with MALDI TOF/TOF mass-spectrometry is proposed. Identification of acylated with succinic acid lysins of fluorescent protein is used as example of such modification identification. Acylation of lysin residue make protein bond cleavage after such lysin with trypsin impossible. Comparison of tryptic proteins of modified and unmodified protein allows to define modification spot.