Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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INFLUENCE ON NITRATES AND NITRITES BIOFILM FORMATION STRAINS PSEUDOMONAS AERUGINOSA WHEN CULTURED ON A LIQUID SYNTHETIC MEDIUM STIMULATES THE FORMATION EXOPOLYMERIC MATRIX

Malinov E.S. 1 Shestakov A.G. 1 Semjonov A.M. 2 Molofeeva N.I. 1 Pulcherovskaja L.P. 1 Karamysheva N.N. 1 Sverkalova D.G. 1 Batrakov V.V. 3 Vasilev D.A. 1
1 Ulyanovsk State Agricultural Academy.a. P.A. Stolypin
2 Moscow State University a. M.V. Lomonosov
3 Ulyanovsk State Pedagogical University a. I.N. Ulyanov
Studied the possibility of different strains of Pseudomonas aeruginosa exopolymeric matrix form on synthetic liquid medium in the presence of potassium nitrate and sodium nitrate, as well as sodium nitrite, under aerobic and anaerobic conditions. During the experiment, we used was 5 strains Pseudomonas aeruginosa. Cultivation of the bacteria Pseudomonas aeruginosa was performed in a liquid synthetic medium, which was replaced included in the composition of the medium L-arginine, potassium nitrate, sodium nitrate, and then, further with sodium nitrite. Cultivation was performed in an oven for 120 hours at 37C. The cultivation of Pseudomonas aeruginosa strains was performed in tubes in both aerobic and anaerobic conditions. The experiment found that strains of Pseudomonas aeruginosa, except №128 capable of forming a exopolymeric matrix under aerobic conditions in a liquid synthetic medium in the presence of potassium nitrate and sodium nitrate and sodium nitrite. Under anaerobic conditions, this ability they lack.