Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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ISOLATION AND STUDY OF BASIC PROPETIAS LIPASE PRODUCING MICROORGANISMS

Orlova S.N. 1 German N.V. 1 Vladimtseva I.V. 1 Kolotova O.V. 1 Boikova I.S. 1
1 Volgograd State Technical University
From the supernatant of activated sludge municipal wastewater treatment plants, flush with industrial grinder Volgograd slaughterhouse and wastewater Volgograd tannery on selective nutrient media conducted allocation of 30 lipolytic bacterial strains. Based on the study oxidation fat ability broth Stern selected three cultures, investigated their basic culture, morphological and lipolytic properties. On selective medium revealed lipase producing bacteria most active strain, carried his identification, which allowed culture attributed to the genus Bacillus. Cloned, mutagenesis and selection selected bacterial isolates. Obtained by high-yielding clone, promising for further research to establish the bacterial preparation, effectively decomposing grease wastewater.