Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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PARCHMENT MANUFACTURE WASTES AS A SUBSTRATE FOR THE CARBOHYDRASE ENZYME COMPLEX PRODUCTION

Osipov D.O. 1 Nemashkalov V.A. 2 Koshelev А.V. 2 Merzlov D.A. 1, 3 Korotkova O.G. 1
1 A.N. Bach Institute of biochemistry of RAS
2 G. K. Skryabin Institute of biochemistry and physiology of microorganisms
3 Lomonosov Moscow State University
It was shown that a parchment could be used as an alternative substrate instead of microcrystalline cellulose for production carbohydrase enzyme complexes secreted by Penicillium verruculosum. Application of parchment in the fermentation medium resulted in reduced activity values and protein content but not more than 25% compared to the control fermentation medium. This increases the lag phase of growth of the fungus 2-fold compared to microcrystalline cellulose due to recalcitrance of the parchment. Therefore, fungal fermentation becomes more expensive. Procedure of parchments enzyme pretreatment was developed. It allows to decrease lag-phase and the fermentation time in 2-fold: it ends after 48 hours from the start of the fermentation. Pretreatment of parchment intended for fungi cultivation by carbohydrase enzyme preparation prior to fermentation resulted in a peak of activity in the culture liquid after 120h ours. With continued culturing enzyme activity values fall.