Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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LC-MS ANALYSIS OF ANTIMICROBIAL PEPTIDES FROM LACTOBACILLUS PLANTARUM 8РА-3

Soboleva A.V. 1 Kolobov A.A. 1 Grishina T.V. 1
1 Saint-Petersburg State University
The mostly potent against standard test-cultures strain L. plantarum 8PA-3 was chosen for purification and characterization of antimicrobial peptides. Low-molecular weight antimicrobial peptides were extracted from culture L. plantarum 8PA-3 using low-pH extraction and ultrafiltration. Fractionation of peptides were performed using cation-exchnge chromatography and reverse-phase high performance liquid chromatography. For testing antimicrobial activity of peptide fractions were used radial diffusion and overlay methods. Spectrum of cationic peptides and the purity of these samples was evaluated using acidic acid electrophoresis and electrophoresis in denaturing conditions. During LC-MS analysis of mostly active fraction of peptides (molecular weights 1 - 10 kDa) were found some peptides with MW: 2089,1 Da; 2112,9 Da; 2293,3 Da and 4845,8 Da.