Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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MODULATORY ROLE OF PRESYNAPTIC A2A-TYPE ADENOSINE RECEPTORS IN TRANSMITTER RELEASE IN MOUSE NEUROMUSCULAR JUNCTIONS

Tarasova E.O. 1 Gaydukov A.E. 1 Balezina O.P. 1
1 Lomonosov Moscow State University
Electrophysiological research revealed that the block of A2A-type adenosine receptors by their selective antagonist ZM241385 doesn’t alter transmitter release during short rhythmic trains (50 Hz during 1 second) of end plate potentials (EPP) in mouse neuromuscular junctions. Nevertheless, under these conditions activation of L-type voltage-gated calcium channels through calcineurin inhibition by cyclosporine A (CsA) doesn’t lead to facilitation of evoked acetylcholine (ACh) secretion. A2A-receptor agonist CGS-21680 produces a significant increase of quantal content of each EPP in the train. We demonstrated that when L-type calcium channels are preliminary activated by CsA application, the addition of CGS-21680 doesn’t result in further potentiation of evoked synaptic transmission. The conclusion has been made that the molecular cascade starting from activation of A2A-receptors by endogenous adenosine doesn’t participate in transmitter release modulation, but it is necessary for ACh release facilitation via L-type calcium channel activation.