Degtyareva O.V. 1
Afanasiev V.N. 1
Khechinashvili N.N. 1
Terpugov E.L. 1
1 Institute of Cell Biophysics
The spectral features of L-lysine monohydrochloride and L-glycine in aqueous solution exposed to a low-intense optical radiation were studied by UV-VIS-spectroscopy and optical microscopy. It was shown that these amino acids at concentration0.3-0.5M demonstrated unusual spectral and optical features under a low-intense optical radiation. Different in amplitude absorption peak was observed around ~270 nm, which was proportionally increased with the increasing of concentration and as in the case of glycine with the “aging” or after repeated illumination of aqueous solution. Both amino acid species showed fluorescence emission in the spectral region 300-500 nm under different excitation wavelength. The spectra of liquid L-lysine HCl and L-glycine had multiple excitation and emission peaks, which indicate on the formation of multiple states and /or multiple species in aqueous medium under given experimental conditions. In another series of experiments we observed of a speckle pattern formation as well as development of aggregation or nucleation inside the transparent amino acids solutions using a cw- low-intense laser light at 532 nm or 633 nm. Our studies showed that a small intensity of light may induce a serious perturbation in structure and behavior of biological molecules at local level.