Akimenko Y.V. 1
Kazeev K.S. 1
Kolesnikov S.I. 1
1 Southern federal university
In model experiments influence thermal (180 °C, tindallization) and chemical (fumigation by chloroform and antibiotics) ways of sterilization on change of qualitative and quantitative structure of microorganisms and enzyme activity of the chernozem ordinary is studied. It is established that more essential inhibition of biological activity, than action of chemical factors has effect of temperature factors. Enzymes were steadiest against action of all used ways of sterilization, than soil microorganisms. On degree of inhibition of soil microorganisms studied ways of sterilization settled down in the following row: 180 °C > chloroform > antibiotics > tindallization. By means of high-quality identification established that a variety of strains decreases among the unsterile soil - sterilization by vapors of chloroform and antibiotics - sterilization by temperature. Thermal factors are most effective concerning bacteria, and fumigation by chloroform and antibiotics concerning chernozem micromycetes.