Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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ENCAPSULATION OF HORSERADISH PEROXIDASE AND IMMUNE-ENZYME CONJUGATE FOR USE IN ENZYME IMMUNOASSAY

Kovalkova M.V. 1 Vorobyova O.V. 2 Avanesyan S.S. 2 Filippova A.M. 2 Zharnikova I.V. 3
1 Criminal Expertise Center of MI of Russia for ST
2 FSAEI of HPE North Caucasian Federal University
3 FPHI Stavropol Anti-plague Institute of Rospotrebnadzor
It is developed the way to incorporate the enzyme horseradish peroxidase and peroxide conjugate in consecutive polyelectrolyte adsorption-formed capsules (carrageenan/chitosan) for enzyme immunoassay (EIA). The enzyme and substrate amount was optimized with Statistic V.6.0 when setting the enzyme reaction. The capsulated enzyme maintained 82,5 % of its activity. The immunoglobulin conjugate was incorporated in microcapsules by co-precipitation method with polyelectrolytes (carrageenan/chitosan). The conjugate incorporation in capsules was 23,6%/ The enzyme immunoassay with using the capsulated conjugate was carried out on polystyrene plates in the sandwich-type. The sensivity of the microencapsulated conjugate is the same as that of the usual one, it is 2x105 m.c./ ml (microbial cells/ milliliter) if there are no cross reaction with heterologous strain B. abortus (Brucellaabortus).