Scientific journal
Scientific Review. Biological science
ISSN 2500-3399
ПИ №ФС77-57454

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MOLECULAR-GENETIC ANALYSIS OF REPAIR (APEX1, XPD) AND CELL CYCLE CONTROL SYSTEMS’ (CHEK2, P53) GENE EXPRASSION FOR MISCARRIAGE

Kutsyn K.A. 1 Kovalenko K.A. 1 Mashkina E.V. 1 Shkurat T.P. 1
1 Southern federal university
The early stages of embryonic development are based on the mechanisms that underlie the functioning of several functional groups of proteins and genes encoding them. However, these mechanisms are not studied sufficiently, which significantly complicates the understanding of the laws of interaction of different cell types, individual body systems and the formation of his reactions. During the first trimester of pregnancy, there is an active cell division and fetal cytotrophoblast. Alterations in the expression of genes, involved in the checkpoints and apoptosis, effect on the embryo viability. In this study, we assessed the distribution of repair (APEX1 (MlM*107748), XPD (MIM*126340) and cell cycle control system СНЕК2 (MIM*604373), P53 (MIM*191170). Estimation of expression level of analyzed genes was performed by RT-qPCR method. In the issue of chorionic tissue analysis decreasing of XPD gene expression level was revealed in comparison to normal pregnancy course (р = 0,003). In miscarriage decidual cells in tissue level expression СНЕК2 lower compared with corresponding samples of chorionic tissue (p = 0,039). Expression level of analyzed genes is lower in the decidua then in the chorion both normal and pathological pregnancy (р = 0,01).